Development of a molecular marker based on chloroplast gene for specific identification of Korean Hibiscus (Hibiscus syriacus ‘Simbaek’)

Abstract Due to the rise in substitution and adulteration of herbal products, as well lack genetic information on Hibiscus plants, more molecular markers are needed understand diversity avoid their misidentification. There many allelic variants functional genes other which control respective phenotypes characteristics. Identifying alleles desired trait by determining diversification through gene-typing allele-specific for authentication is, therefore, a potent strategy. The purpose this study was use insertion/deletion (InDel) identify syriacus cultivars. We developed novel InDel marker Korean ‘Simbaek’, based trnL-F region chloroplast gene. Through site, modified specific primer pair multiplex polymerase chain reaction (PCR) system were identification Simbaek cultivar. highly accurately authenticated from five main cultivars H. . Taken together, described method is potential tool selection germplasm resource Graphical